Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found. Petroleum microbiology is a branch of microbiology that deals with the study of microorganisms that can metabolize or alter crude or refined petroleum products. These microorganisms, also called hydrocarbonoclastic microorganisms, . ” Most Hydrocarbonoclastic Bacteria in the Total Environment are Diazotrophic, which. The marine hydrocarbonoclastic bacterium Alcanivorax borkumensis is able to degrade mixtures of n-alkanes as they occur in marine oil spills. However.

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The isolate with the best COD reduction capability is P. Although, maximum efficacy were observed using gram positive bacteria which is contrary to our report of gram negative bacteria.

Incubation was for 2 weeks. The toxicity of hydrocarbon can result in cancer, gastrointestinal infection, heart disease, neurological disorder and respiratory infection Leachy and Rita, The percentage loss of total peak areas in the GLC chromatograms based on the peak areas of the control samples similarly prepared, but using previously autoclaved bacteria was calculated as a quantitative measure of oil consumption.

Therefore their sampling is always done from hydrocarbon contaminated sites.

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All five bacterial isolates manifested no lag phase of growth. The two general strategies for enhancing oil recovery are altering the surface properties of the interface and using bioclogging to change the flow behavior. Agriculture Bioinformatics Applications Biotech Products.

It is experimentally challenging to unequivocally demonstrate diazotrophy in the 82 different bacterial species that gave positive presumptive tests. On the other hand, these bacteria only grew and consumed crude oil in the NaNO 3 -free medium when the top space gas contained nitrogen. This result implies that the gram negative bacteria are more effective for degradation of organic compounds than the gram positive bacteria. Dion P, Nautiyal CS, editors. Crude oil-consumption by bacterial species in the presence and absence of NaNO 3 in the medium and nitrogen gas in the culture top space.


Petroleum effluent is discharged into River Rido and this poses a threat to the communities that utilize the water downstream of River Rido, the ultimate recipient of the effluent. Hydrocarbon degradation in marine habitat is largely dependent on temperature, salinity, depth, latitude and redox potential. Author Spotlight Raghuveer Singh. To control such risk, bioremediation constitutes an environmentally friendly alternative technology that has been established and applied.

Alternatively, oil-mobilizing products can be produced by fermentation and hydrocarboniclastic into the reservoir. In the simplest case, indigenous microbial communities can degrade the petroleum where the spill occurs. There are two ways by which biosurfactants are involved in bioremediation. Degradation of aliphatic hydrocarbons.

Oil-bioremediation potential of Arabian Gulf mud flats rich in diazotrophic hydrocarbon-utilizing bacteria.

Petroleum microbiology – Wikipedia

Regarding isolations from sea water and hypersaline areas, 0. Join as an Editorial Member. Details of the culture sources and methods for bacterial isolation and characterization have been described in our previous study 1. Potential Hydrocarbonoclastic Bacteria By: Each flask was inoculated with 0. Physical and chemical approaches are capable of removing a broad spectrum of contaminants but the main disadvantage of these methods lie in the increased energy consumption and the need of additional chemicals Godheja et al.

Emulsifiers produced by microbes can break up oil into smaller droplets, effectively increasing the available surface area. A previous study estimated that 60 mg Bavteria was needed for the microbiological consumption of 1 g hydrocarbons 9. This finding is similar to the study carried out by Kafilzadeh et al. More Bookmarks Digg etc.

Nitrogen fixation by the most frequently bacterix bacterial species Table 1 was confirmed from a novel experiment designed for this purpose. Furthermore, we only selected one of the two Pseudomonas sp. Other important characteristics of HCB are that they have very small genome, few rRNA operons and cytoplasm contains very small number of proteins. The gel electrophoresis hydrocarbonoclastiic in Fig.

The results obtained is relatively lower than that of Abdullahi et al. Hydrocarbons are principle constituents of petroleum, related byproducts like coal tar, diesel, petrol, gasoline, kerosene and major environmental pollutants.


The predominant bacterial detected by both dependent and independent approaches were the Proteobacteria. According to Ornston and Yehthe utilization of hydrocarbon by bacteria is feasible as they possess catabolic enzymes for hydrocarbon metabolism and more importantly they have an immense capacity for adaptive change. On the other hand, we subjected Pigmentiphaga daeguensis to this analysis Table 2 even though it was not among the species in Table 1.

Hydrocarbon-utilization by nodule bacteria and plant growth promoting rhizobacteria. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Sphingomonas dokdonensis 1 and Marinobacter M. Frontiers in Environmental Microbiology.

Petroleum microbiology

Published online Feb 7. Attenuation of oil pollutants in the Arabian Gulf water by bacteria naturally associated with live fish.

Oil-utilizing bacterial species from oil-contaminated sites in Kuwait selected for this study. Hydrocarbons are compounds whose structures hydrocarboonclastic made up of hydrogen and carbon atoms. Eighty-two out of the hydrocarbonoclastic bacterial species tested were capable of growing on the Htdrocarbonoclastic 3 -free mineral medium with oil as the sole source of carbon and energy. The mineral medium used was provided with 0. By using this site, you agree to the Terms of Use and Privacy Policy.

Bioremediation is the use of biological agents such as microorganisms bacteria, fungi and algae and plants for the removal or detoxification of toxic substances in the environment. Isolation of Hydrocarbonoclastic Bacteria 2. Hydrocarbonoclastic potential of HCB in freshwater is dependent on emulsification or surfactant activity of strains, pH, concentration of contaminant in that habitat.

Five possible hydrocarbonoclastic bacteria from the refinery effluent were identified morphologically and biochemically as Pseudomonas aeruginosa, Proteus vulgarisKlebsiella hydrocarbknoclastic, Serretia marcescens and Streptomyces sp.