Review Article necessary and various intracanal medicaments have been tried. Intracanal medicaments, calcium hydroxide, antibiotics, corticosteroids. free root canal systems, especially in pulpless teeth, it is necessary to use intra- canal medicaments. These medicaments must be capable of destroying bacteria . Request PDF on ResearchGate | Antibiotics as intracanal medicaments: a review | Antibiotics are an extremely valuable addition to the armamentarium.

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The purpose of this paper is to discuss critically the antibacterial efficacy of intracanal medicaments on bacterial biofilm. The selected articles were identified from titles, abstracts and full-text articles by two independent reviewers, considering the tabulated inclusion and exclusion criteria. Disagreements were resolved by consensus. The search retrieved 91 related articles, of which 8.

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Intracanal medicaments were found to have a limited action against bacterial biofilm. Intraacanal principle of a treatment to reach favorable outcomes in endodontic infection management requires the recognition of the problem and the removal of the etiological factors. The microenvironment of root canal presents excellent conditions to establish microbial growth The success of infected root canal therapy is influenced by discrepancy gradients of microorganisms between the endodontic biofilm and the planktonic suspension The phases for the microbial community to develop a biofilm and colonize the environment may sometimes be unusual, but basically occur with the same sequence of developmental steps: Reduction of endodontic intracansl has been achieved by a series of antimicrobial strategies that include root canal preparation, irrigating solutions, intracanal dressing, and root canal filling.

However, innumerous published articles on endodontic infection control are motive of controversy 19 – 111619242635 Several studies have been developed with different bacterial biofilm models to test the antimicrobial effectiveness of endodontic medicaments 19 – 111619242635 Investigations have shown either antimicrobial success 9 or failure 51130 of intracanal medicaments on bacterial biofilm, as well as the presence of biofilm on the entire external root apex surfaces in teeth mediaments pulp necrosis and radiographically visible periapical lesions The susceptibility of oral bacterial biofilm to antimicrobial agents indicates the need to reproduce in vivo conditions where bacteria grow as biofilm on tooth surfaces Evidence-based dentistry involving systematic reviews or meta-analyses has been the ultimate goal to integrate the best available research evidence with clinical experience.

In this context, it medicametns important to make a critical analysis of therapeutic protocols for the management of bacterial biofilm. The requirement for its development includes studies in human subjects with definite clinical issues, using a critical longitudinal analysis of published articles.

The focus on answering clinical questions can be structured by means of problem, intervention, comparison and outcome. Uncountable investigations 1 – 57912 – 1518202223revidw2731 – 34383944 about the antimicrobial intracanql of intracanal medicaments on bacterial biofilm have shown contradictory results, but the clinical question on how to eliminate the endodontic bacterial biofilm remains unclear.

The efficacy of a particular agent was dependent on the nature of the organism in the biofilm and on the contact time. NaOCl was generally the most effective agent followed by iodine. However, the clinical effectiveness of these agents must be evaluated in the light of the complexity of root canal anatomy and polymicrobial nature of root canal infections. The difference in gradients of bacterial killing amongst the biofilm, planktonic suspension or pellet presentation was significant and dependent upon the agent, except for NaOCl and calcium hydroxide, in which no difference could be detected.

The irrigation solutions tested for 20 min were not sufficient to inactivate E. Relevant clinical considerations are yet to be elucidated, such as the efficacy of intracanal medicament on bacterial biofilm, the time necessary for a mature endodontic biofilm to form or the ideal biofilm model to assess antimicrobial endodontic substances.

The purpose of this paper is to make a critical analysis of the published research on the antibacterial efficacy of intracanal medicaments on bacterial biofilm. This work was developed based on an analysis of longitudinal studies from a quantitative systematic review of results of several investigations.

English-language articles were’ retrieved from electronic biomedical journal databases and handsearching records. Prospective studies were reviewed with regard to the efficacy of the intracanal medicaments on bacterial biofilm. The following databases were searched on August 1 reeview of For the electronic search strategy the terms presented in Table 1 were used as keywords in several combinations.

Handsearching was conducted by the review of the reference lists of the eligible clinical trials and the review of author’s personal databases of trial reports in an attempt to identify any other relevant studies.


Table 2 shows the inclusion and exclusion criteria for selection of the articles. Based on the inclusion and exclusion, 91 articles were selected, of which 17 None of the 8 articles referring to in vivo studies met all inclusion criteria Table 3.

Table 4 describes in vitro research articles that were excluded for referring to different bacterial biofilm models, contamination time, root canal cleaning and shaping techniques, and therapeutic efficacy. No article selected in a preliminary review by handsearching met the inclusion criteria either. The results of the analysis demonstrated inefficacy of intracanal medicaments on bacterial biofilm. Systematic reviews, associated or not to meta-analyses, represent major positions at the evidence pyramid towards a direct decision making, capable to indicate clinical procedures certificated by more trustable arguments.

The major difficulty found in the present study was the great deal of available information, and the large number of investigations with agreeing and disagreeing conclusions. Scientifically based evidence that confirms the efficacy of intracanal medicaments on bacterial biofilm involves a profound reflection and discussion, regardless of the limits and caution with the methodology 16172136 Longitudinal studies signalize that there is yet no accurate solution to eliminate or disrupt the root canal bacterial biofilm.

However, the results of this critical review confirmed that root canal preparation, with careful disinfection and use of intracanal substances that provide good antimicrobial efficacy, tissue dissolution capacity, and acceptable biocompatibility, will definitely improve the prognosis of the treatment of apical periodontitis 25 From a total of 91 articles, 8.

Interestingly, a recently published study contemplating the clinical aspects about the bacterial biofilm is the only one with high level of evidence.

The findings highlight the importance and need for stringently applying non-antibiotic chemomechanical measures in order to disrupt the biofilm and reduce the intraradicular microbial load to the lowest possible level to ensure the most favorable long-term prognosis for treatment of infected root canals.

In accordance with this study, other investigations also recovered microorganisms from teeth with endodontic infection after antimicrobial endodontic therapy 6 The findings of in vitro studies Table 4 show an antimicrobial effectiveness of intracanal medicaments on bacterial biofilm 139 similarly to in vivo evaluations involving the presence of biofilm on all external root apex surfaces in teeth with pulp necrosis with periapical lesions Most studies about endodontic biofilm are in vitro experiments.

Therefore, care should be taken to avoid inappropriate extrapolation of results, which does not allow a clinical decision for therapeutic protocols in humans. It is mandatory to consider the role and importance of biofilm in endodontics, as well as its definition.

In the present study, it was not possible to obtain an ideal combination of results, considering the high heterogeneity of the adopted clinical protocols. This fact limited the performance of a meta-analysis. The large number of published papers can bring in its context contradictory conclusions. The variability between the employed methodologies, study selection, publication biases, absence of randomized controlled trial, access to all the information of the published experiments and the own nature of the essays, signalizes for some critical implications towards the equation of the problem.

A clinical question that supports the decision about the antibacterial efficacy of intracanal medicaments on bacterial biofilm cannot be answered using a critical longitudinal analysis of published articles. Various obstacles on the analysis of the pertinent data for the included studies involved methodological data that were hidden and that present a relative degree of importance.


Relevant aspects of the medicament that met the inclusion criteria were considered, such as the experimental model and sample size, the time lapsed from the initial endodontic treatment until the present moment, the method of identification of the bacteria, the presence of bacteria on the initial samples, the chemical substances used during the root canal disinfection, the time of maintenance of the intracanal dressing prior to obturation, and medicxments efficacy of the medicaments against the tested bacteria.

However, the difficulty for comparing these revkew was due to differences in the adopted treatment strategies, as follows: Despite all known limitations, studies that intracaanl not developed in humans are also relevant because, prior to clearance for human use, biological evaluations of dental materials, initial and secondary tests are required Other aspects that must be critically observed and judiciously analyzed are the biofilm model to test the antimicrobial endodontic medicaments, the biological indicator, and the time necessary for biofilm formation.


Socransky and Haffajee 37 reported that the reason for the existence of a biofilm is that it allows microorganisms to stick to and multiply on surfaces. Thus, attached bacteria sessile growing in a biofilm display a wide range of characteristics that provide a number of advantages over single-cell planktonic bacteria. A major advantage is the protection that the biofilm provides to colonizing species from microorganisms competing for environmental factors, such as host defense mechanisms, and from potentially toxic substances in the environment, such as lethal chemicals or antibiotics.

Biofilms can also facilitate processing and uptake of nutrients, cross-feeding one species providing inrtacanal to anotherremoval of potentially harmful metabolic products often used by other bacteria as medica,ents as the development of an appropriate physicochemical environment such as a properly lowering the oxidation reduction potential. Bacterial biofilm has an open architecture with channels traversing from the biofilm surface.

This structure affects the movement of molecules and gradients in key determinants develop.

Bacteria growing on a surface display a novel phenotype, one of the consequences being an increased resistance to antimicrobial agents. Resistance can result from restricted inhibitor penetration, slower bacterial growth rates, transfer of resistance genes, suboptimal environmental conditions for inhibitor activity, and the expression of a resistant phenotype Duggan and Sedgley 10 tested the hypothesis that the ability of E.

The variations observed in these clinical isolates suggested that biofilm formation might be an important factor when considering the virulence phenotype of endodontic strains in general. Changes in the environment, such as calcium hydroxide-related pH increase or the effect of antimicrobials, are capable of triggering genetic cascades that modify the physiological characteristics of bacterial cells.

Antibiotics as intracanal medicaments: a review.

Surface adherence by bacteria to form biofilms is a good example of bacterial adaptation. More information is now available about the existence of polymicrobial biofilm communities on root canal walls coupled with new data showing that the adaptive mechanisms of bacteria in these biofilms medivaments significantly augmented for increased survival.

This ecological view on the persisting intracanl problem in endodontics suggests that the action of individual species in refractory endodontic infections is secondary when compared to the adaptive changes of a polymicrobial biofilm community undergoing physiological and genetic changes in response to changes in the root canal environment.

The invasion of root dentinal tubules by root canal bacteria is a multifactorial event in which a limited number of oral bacterial species have the required properties to participate The penetration of microorganisms in infected root dentin have shown variations considering the experimental model, the biological indicator and the time of incubation employed in the studies Table 4.

In order to develop new treatments to eradicate E. For all reasons addressed in this paper, and considering the heterogeneity of guidelines to study antimicrobial strategies for endodontic infections and the high clinical success estimate, adequate disinfection assisted by the intracanal medicaments reduces the bacterial population and favors the prognosis. The antimicrobial efficacy of intracanal medicaments on bacterial biofilm still needs to be confirmed. Further studies are required to offer new guidelines for the treatment protocol of endodontic biofilm.

National Center for Biotechnology InformationU. J Appl Oral Sci. Author information Article medicamentz Copyright and License information Disclaimer. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Abstract The purpose of this medciaments is to discuss critically the antibacterial efficacy of intracanal medicaments on bacterial biofilm. Biofilm, Intracanal medicaments, Antimicrobial efficacy. Open in a separate window. Study Selection The selected articles were identified from titles, abstracts and full-text articles by two independent reviewers, considering the tabulated inclusion and exclusion criteria.

Inclusion criteria In vivo studies, in humans. Susceptibilties of two Enterococcus faecalis phenotypes to root canal medications. Yag laser irradiation of the microbiological apical biofilm. Coronal leakage of mixed anaerobic bacteria after obturation and post space preparation. Bactericidal effect of Nd: YAG laser irradiation on some endodontic pathogens ex vivo.

Response to alkaline stress by root canal bacteria in biofilms. Bacteria recovered from teeth with apical periodontitis after antimicrobial endodontic treatment. The Effect of Exposure to irrigant solutions on apical dentin biofilms in vitro.